Bridge RNAs direct programmable recombination of target and donor DNA
Researchers discovered a novel Bridge RNA mechanism enabling precise DNA recombination. The IS110 system allows programmable DNA rearrangements, offering insights into genetic diversity and genome design beyond CRISPR and RNA interference.
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Researchers have discovered a novel mechanism involving Bridge RNAs that direct programmable recombination of target and donor DNA. The study focuses on IS110 insertion sequences, which express a structured non-coding RNA binding specifically to their encoded recombinase. This Bridge RNA contains two internal loops that base-pair with target DNA and donor DNA, allowing for sequence-specific recombination between two DNA molecules. The modularity of this system enables DNA insertion into genomic target sites, programmable DNA excision, and inversion. The IS110 bridge recombination system offers a unified mechanism for fundamental DNA rearrangements required for genome design, expanding the diversity of nucleic-acid-guided systems. The study sheds light on the role of non-coding RNAs in facilitating DNA recombination and highlights the potential for manipulating DNA sequences with high specificity. This research provides insights into the mechanisms underlying genetic diversity and genome design, offering a new perspective on DNA rearrangement processes beyond existing systems like CRISPR and RNA interference.
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