Bridge RNAs direct programmable recombination of target and donor DNA
Researchers discovered a novel Bridge RNA mechanism enabling precise DNA recombination. The IS110 system allows targeted DNA insertion, excision, and inversion, offering insights into genetic diversity and genome manipulation for genetic engineering.
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Researchers have discovered a novel mechanism involving Bridge RNAs that direct programmable recombination of target and donor DNA. The study focuses on IS110 insertion sequences, which express a structured non-coding RNA binding specifically to their encoded recombinase. This Bridge RNA contains two internal loops that base-pair with target DNA and donor DNA, allowing for sequence-specific recombination between two DNA molecules. The modularity of this system enables DNA insertion into genomic target sites, programmable DNA excision, and inversion. The IS110 bridge recombination system offers a unified mechanism for fundamental DNA rearrangements required for genome design, expanding the diversity of nucleic-acid-guided systems. The study sheds light on the role of non-coding RNAs in facilitating DNA recombination and highlights the potential for manipulating DNA sequences with high specificity. This research provides insights into the mechanisms underlying genetic diversity and genome design, offering new possibilities for genetic engineering applications.
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Binrw
The tool binrw simplifies binary parsing and serialization with a declarative approach, offering readability and maintainability. It supports common tasks, generics, custom parsers, predefined types, and is safe for various environments.
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Modern processors use branch predictors like RAS to boost performance by predicting control flow. Microbenchmarking on Intel and AMD processors reveals RAS behavior, accuracy, and limitations, emphasizing accurate branch prediction for high performance.
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Researchers discovered a novel Bridge RNA mechanism enabling precise DNA recombination. The IS110 system allows programmable DNA rearrangements, offering insights into genetic diversity and genome design beyond CRISPR and RNA interference.
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Researchers at the University of Tokyo used DIPA-CRISPR to edit tardigrade genes, aiming to understand resilience mechanisms. This technique could have medical applications, like preserving human organs. The study reveals tardigrades' genetic traits.