DNA-based bacterial parasite uses new DNA-editing method
Researchers found a new DNA parasite, IS110, using unique DNA-editing to move in bacterial genomes. It inserts DNA with non-protein-coding RNAs as guides, showing potential for gene editing despite high error rates. Refinement needed for safety and accuracy in vertebrate genomes.
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Researchers have discovered a new DNA-based parasite that utilizes a unique DNA-editing method to move around bacterial genomes. This parasite, known as IS110, forms a circular DNA structure with specific sequences that trigger the production of non-protein-coding RNAs. These RNAs act as guides, ensuring precise insertion of the parasite's DNA into specific locations in the genome. The researchers demonstrated the potential of this system for gene editing by directing the insertion of DNA sequences and causing specific deletions in the genome. However, the method's low specificity, with recognition sequences as short as four to seven bases, resulted in high error rates ranging from 50% to 94%. While the system shows promise for gene editing, further refinement is necessary to improve its accuracy before it can be considered safe for practical applications. The researchers suggest that extending the recognition sequences could enhance specificity, but more research is needed to optimize this DNA-editing method for potential use in vertebrate genomes.
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Researchers discovered a novel Bridge RNA mechanism enabling precise DNA recombination. The IS110 system allows programmable DNA rearrangements, offering insights into genetic diversity and genome design beyond CRISPR and RNA interference.
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